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KMID : 0900920000240030281
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Korean journal of Animal Reproduction
2000 Volume.24 No. 3 p.281 ~ p.288
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Studies on Oocyte Activation Regimen for Nuclear Transfer in Hanwoo(Korean Cattle)
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Yang B.-S.
Park Chang-Sik
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Abstract
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This experiment was carried out to investigate the optimal activation condition for parthenogenetic development. In order to activate oocytes at 22 h post onset of maturation, the oocytes were subjected to 5 muM ionomycin(I) for 5 min ,10 muM calcium ionophore(Ca) for 5 min, 2 mM 6-dimethylamino-purine(DMAP) for 3 h and 10 mutextrm{g}/mell cycloheximide(CH) for 6 h alone or in combination. The activated oocytes were cultured in modified CR_1aa at 5% CO_2, 5% O_2, 90% N_2. l. The cleavage rates after 48 h culture of oocytes treated with I, Ca, DMAP and CH were 12.7%, 14.1%, 28.9% and 22.9%, respectively. There was no blastocyst formation. 2. The cleavage rates after 48 h culture of oocytes treated with I ＋ DMAP, I ＋ CH, Ca ＋ DMAP and Ca ＋ CH were 96.9%, 82.1%, 93.1% and 34.7%, respectively. Developmental rates to blastocysts were 10.4%, 5.3%, 17.6% and 7.1 %, respectively. When oocytes were treated with Ior Ca followed by DMAP, the blastocyst formation rate was significantly higher than other groups(P <0.05). 3. According to single activation treatment, pronucleus formation rates were 5.4%, 3.6%, 28.3% and 28.8%, respectively, Whereas, all oocytes treated with the combined activation agents formed 100% pronucleus.
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KEYWORD
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Parthenogenetic development, Cleavage rates, Activation agents
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